Table 2 |
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|
Functional siRNA screening data for 11 GBM survival-associated genes in the A172 glioblastoma cell line |
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|
Symbol |
Description |
siRNA name |
CTG |
Caspase |
Survival |
Expression |
|
|
||||||
|
AllStars Cell Death Vontrol siRNA (Cell death ctrl) |
-13.80 |
19.62 |
NA |
NA |
||
|
AllStars Negative Control siRNA (siNEG) |
0.00 |
0.00 |
NA |
NA |
||
|
KIF11 |
Kinesin family member 11 |
KIF11_7 |
-9.56 |
7.39 |
NA |
NA |
|
PLK1 |
Polo-like kinase 1 |
PLK1_7 |
-5.92 |
0.31 |
NA |
NA |
|
FLNC |
Filamin C, gamma |
FLNC_2 |
2.56 |
-0.89 |
0.000189 |
2.51 |
|
FLNC_5 |
-0.37 |
0.29 |
||||
|
FLNC_6 |
-0.57 |
-0.94 |
||||
|
FLNC_7 |
-5.39 |
0.49 |
||||
|
H19 |
H19, imprinted |
H19_1 |
0.38 |
-0.14 |
0.000588 |
3.54 |
|
maternally expressed |
H19_2 |
-5.08 |
1.17 |
|||
|
transcript (non-protein |
H19_3 |
5.20 |
-2.71 |
|||
|
coding) |
H19_4 |
-2.97 |
2.71 |
|||
|
HIST1H4L |
Histone cluster 1, H4l |
HIST1H4L_1 |
1.63 |
-0.60 |
0.001560 |
5.01 |
|
HIST1H4L_2 |
2.61 |
-2.00 |
||||
|
HIST1H4L_5 |
-0.36 |
-1.19 |
||||
|
HIST1H4L_7 |
-5.93 |
-0.97 |
||||
|
KIAA0040 |
KIAA0040 |
KIAA0040_11 |
-2.94 |
-0.64 |
0.000867 |
2.63 |
|
KIAA0040_12 |
0.63 |
-0.83 |
||||
|
LOC100129443_3 |
0.69 |
-0.71 |
||||
|
LOC100129443_4 |
-0.31 |
-0.30 |
||||
|
LTF |
Lactotransferrin |
LTF_1 |
-1.74 |
0.22 |
0.001570 |
3.95 |
|
LTF_2 |
-0.03 |
0.07 |
||||
|
LTF_5 |
-1.04 |
0.44 |
||||
|
LTF_6 |
-1.18 |
-0.79 |
||||
|
NNMT |
Nicotinamide N- |
NNMT_5 |
-0.91 |
-1.29 |
0.000074 |
7.41 |
|
methyltransferase |
NNMT_6 |
-0.48 |
-1.87 |
|||
|
NNMT_7 |
-1.26 |
-0.32 |
||||
|
NNMT_8 |
-0.30 |
-0.10 |
||||
|
POSTN |
Periostin, osteoblast |
POSTN_1 |
0.24 |
-1.16 |
0.001950 |
14.9 |
|
specific factor |
POSTN_2 |
0.28 |
-2.11 |
|||
|
POSTN_6 |
-2.17 |
0.56 |
||||
|
POSTN_7 |
0.52 |
0.12 |
||||
|
TAGLN2 |
Transgelin 2 |
TAGLN2_10 |
4.53 |
-1.74 |
0.001010 |
4.21 |
|
TAGLN2_11 |
-3.78 |
0.48 |
||||
|
TAGLN2_8 |
-0.43 |
0.45 |
||||
|
TAGLN2_9 |
4.67 |
-1.94 |
||||
|
TIMP1 |
TIMP metallopeptidase |
TIMP1_2 |
-0.09 |
-0.96 |
0.000109 |
3.21 |
|
inhibitor 1 |
TIMP1_4 |
1.07 |
0.88 |
|||
|
TIMP1_5 |
0.56 |
-1.87 |
||||
|
TIMP1_6 |
-0.08 |
0.41 |
||||
|
MSN |
Moesin |
MSN_8 |
-1.68 |
1.61 |
0.000028 |
3.42 |
|
MSN_9 |
-4.49 |
-1.05 |
||||
|
MSN_5 |
-0.36 |
-1.57 |
||||
|
MSN_1 |
-2.57 |
0.80 |
||||
|
CDKN2A |
Cyclin-dependent kinase inhibitor 2A |
NA (gene deleted in A172) |
||||
|
|
||||||
|
Cell proliferation (CTG) and induction of caspase-3 and -7 activities (Caspase) were assayed after transfection of A172 cells with four siRNAs against each gene. Z-scores from the proliferation and caspase-3/7 assays are presented, centered on the scramble siRNA. Values in bold diverge by more than two standard deviation units from the median of scramble negative control siRNA and are considered significant. For each gene, the best survival P-value (Survival) and the corresponding fold change in the exon array (Expression) are given. |
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|
Ovaska et al. Genome Medicine 2010 2:65 doi:10.1186/gm186 |
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