Additional file 4.

Cytotoxicity assays for transfection of pre-miRs and anti-miRs. MTS cell proliferation assays (Promega) were conducted to determine the number of viable cells in three nonautistic LCLs after transfection with (a) 30 nM pre-miRs, or (b) 30 nM anti-miRs, for 72 hours. No significant cytotoxicity was found under any transfection condition.

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Sarachana et al. Genome Medicine 2010 2:23   doi:10.1186/gm144