Table 4

Overview of in vivo delivery systems for snoRNAs and miRNAs*

miRNA

Disease state

Biological target

In vivo model

Delivery system

References

miR-100

Nasopharyngeal cancer

Plk1

6- to 8-week-old SCID BALB/c female mice

siRNA and ionizing radiation and potential miRNAs that might regulate Plk1 expression

[88]

Synthetic miR-16

Metastatic prostate cancer

CDK1 and CDK2

Bone metastasis mode mice

Injected into tail veins

[89]

miR-155

Myeloproliferative disorder

SHIP1

Mice, specific knockdown of SHIP1 in the hematopoietic system

Retroviral delivery of a miR-155-formatted siRNA against SHIP1

[90]

SNORD116 (PWCR1/HBII-85)

Prader-Willi syndrome

Bioinformatic screen located 23 possible targets

C57BL/6 mice

Snord116del mice

[21]

miR-147

Inflammation

Cytokine expression in macrophages stimulated with ligands to Toll-like receptors: TLR2, TLR3, TLR4

LPS-stimulated mouse

Peritoneal macrophages were transfected with 40 nM control miRNA mimics or mouse miR-147 mimics

[91]

LNA-antimiR-122

Hypercholesterolemia

miR-122 liver specific

Normal and hypercholesterolemic mice; normal African green monkeys

Intravenous injections unconjugated

[62]

LNA-antimiR-21

Glioma

miR-21

Athymic nude mice

Intracranial cell implantation

[92]

miR-34a

Normal

E2F family

Nude mice

Subcutaneous administration of miR-34a/collagen complexes

[93]

mir-17-92 cluster

Normal

c-Myc

Mice reconstituted with hematopoietic stem cells expressing mir-17-19b

Perfusion cells treated by retroviral vector

[94]

*Abbreviations: CDK, cyclin-dependent kinase; E2F, E2 transcription factor; LPS, lipopolysaccharide; Plk1, Polo-like kinase 1; SCID, severe combined immunodeficient; SHIP1, Src homology-2 domain-containing inositol 5-phosphatase 1; TLR, Toll-like receptor.

Galasso et al. Genome Medicine 2010 2:12   doi:10.1186/gm133