Table 3

Genes correlating with weight loss

Center 1 (n = 21)

Center 2 (n = 13)

Gene-chip

RT-qPCR

Gene

CC rectus abdominis

CC rectus abdominis

Regression P-value

CC vastus lateralis

Regression P-value

CC diaphragm

Regression P-value

APCDD1

-0.74

-0.51

0.03

0.26

NS

-0.20

NS

CAMk2B

0.82

0.50

0.01

0.45

0.06

0.50

0.03

EIF3I

0.64

0.50

0.02

0.10

NS

0.20

NS

HGS

0.7

0.67

0.00

0.17

NS

0.20

NS

NUDC

0.65

0.72

0.00

0.13

NS

0.0

NS

POLRMT

0.6

0.51

0.02

0.07

NS

0.0

NS

TIE1

0.67

0.53

0.01

0.70

0.003

0.0

NS

TSC2

0.69

0.47

0.03

0.40

0.1

0.0

NS

Significance analysis of microarrays (SAM) identified 82 genes correlating with weight loss. qRT-PCR validated eight of nine selected targets from this list (correlation coefficient (CC)). These eight genes were also examined in the cohort from center 2 using RNA extracted from anatomically distinct regions. For each gene the correlation coefficient from the Affymetrix data set is shown followed by the correlation coefficient for qRT-PCR and a P-value for this latter regression. NS: not significant.

Stephens et al. Genome Medicine 2010 2:1   doi:10.1186/gm122