Table 6

Validation of DKK1, UGT1A and EEF1A1 overexpression in the resistant cells

Expression

Gene

Cell line

Microarray

RT-PCR validation

DKK1

HT29

4.25

5.66 ± 0.23

Caco-2

2.56

1.96 ± 0.03

UGT1A

MCF-7

15.90

23.41 ± 0.94

MDA-MB-468

19.27

16.28 ± 0.19

EEF1A1

MIA PaCa-2

2.29

3.89 ± 0.16

K562

2.75

2.38 ± 0.47

Saos-2

2.05

1.85 ± 0.15

The expression levels for the three node genes (DKK1, UGT1A and EEF1A1) derived from both the microarray data (average of the overexpressed UGT1A family members) and real-time RT-PCR are given. All the results are expressed as fold changes relative to the sensitive cells and values are the mean of triplicate experiments ± SE.

Selga et al. Genome Medicine 2009 1:83   doi:10.1186/gm83